Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in nonadherent cells

Sayers, Edward J. ORCID: https://orcid.org/0000-0002-2621-1119, Barlow, Victoria L., Tsai, Yu-Hsuan ORCID: https://orcid.org/0000-0003-0589-5088 and Jones, Arwyn T. ORCID: https://orcid.org/0000-0003-2781-8905 2022. Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in nonadherent cells. Cell Penetrating Peptides, Vol. 2383. Methods in Molecular Biology book series, Springer, pp. 211-228. (10.1007/978-1-0716-1752-6_14)

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Abstract

Cyclization of cell-penetrating peptides (CPPs) often results in improved capacity for intracellular delivery of a range of cargoes but quantitating the distinct subcellular localization of them, and their linear counterparts, remains a challenge. Here we describe an optimized method for recombinant generation and purification of eGFP attached to the cyclic form of the newly discovered CPP EJP18 in E. coli. We also demonstrate a novel microscopy method for quantifying its subcellular distribution in leukemia cells.

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Chemistry Pharmacy
Publisher:	Springer
ISBN:	9781071617519
ISSN:	1064-3745
Last Modified:	06 Jan 2024 04:33
URI:	https://orca.cardiff.ac.uk/id/eprint/145786

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