Sayers, Edward J.  ORCID: https://orcid.org/0000-0002-2621-1119, Barlow, Victoria L., Tsai, Yu-Hsuan ORCID: https://orcid.org/0000-0003-0589-5088 and Jones, Arwyn T. ORCID: https://orcid.org/0000-0003-2781-8905
2022.
Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in nonadherent cells.
Cell Penetrating Peptides,
Vol. 2383.
Methods in Molecular Biology book series,
Springer,
pp. 211-228.
(10.1007/978-1-0716-1752-6_14)
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Official URL: http://dx.doi.org/10.1007/978-1-0716-1752-6_14
Abstract
Cyclization of cell-penetrating peptides (CPPs) often results in improved capacity for intracellular delivery of a range of cargoes but quantitating the distinct subcellular localization of them, and their linear counterparts, remains a challenge. Here we describe an optimized method for recombinant generation and purification of eGFP attached to the cyclic form of the newly discovered CPP EJP18 in E. coli. We also demonstrate a novel microscopy method for quantifying its subcellular distribution in leukemia cells.
| Item Type: | Book Section |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Chemistry Schools > Pharmacy |
| Publisher: | Springer |
| ISBN: | 9781071617519 |
| ISSN: | 1064-3745 |
| Last Modified: | 06 Jan 2024 04:33 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/145786 |
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