Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in nonadherent cells

Sayers, Edward J., Barlow, Victoria L., Tsai, Yu-Hsuan and Jones, Arwyn T. 2022. Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in nonadherent cells. Cell Penetrating Peptides, Vol. 2383. Methods in Molecular Biology book series, Springer, pp. 211-228. (10.1007/978-1-0716-1752-6_14)

Full text not available from this repository.

Abstract

Cyclization of cell-penetrating peptides (CPPs) often results in improved capacity for intracellular delivery of a range of cargoes but quantitating the distinct subcellular localization of them, and their linear counterparts, remains a challenge. Here we describe an optimized method for recombinant generation and purification of eGFP attached to the cyclic form of the newly discovered CPP EJP18 in E. coli. We also demonstrate a novel microscopy method for quantifying its subcellular distribution in leukemia cells.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Chemistry
Pharmacy
Publisher: Springer
ISBN: 9781071617519
ISSN: 1064-3745
Last Modified: 02 Dec 2021 12:07
URI: http://orca.cardiff.ac.uk/id/eprint/145786

Actions (repository staff only)

Edit Item Edit Item