Analysis of human tumour necrosis factor receptor 1 dominant-negative mutants reveals a major region controlling cell surface expression

Fielding, Ceri Alan ORCID: https://orcid.org/0000-0002-5817-3153, Siebert, Stefan, Rowe, Martin and Brennan, Paul ORCID: https://orcid.org/0000-0001-8792-0499 2004. Analysis of human tumour necrosis factor receptor 1 dominant-negative mutants reveals a major region controlling cell surface expression. FEBS Letters 570 (1-3) , pp. 138-142. 10.1016/j.febslet.2004.06.035

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Abstract

Tumour necrosis factor receptor 1 (TNFR1) plays a critical role in host defence and inflammation. We have identified a membrane proximal region (aa 218-324) of TNFR1 that restricts surface expression. This was prompted by comparing the dominant-negative properties of a C-terminal truncation of TNFR1 with a point mutant that prevents signalling. C-terminal truncation (aa 218-426) generates a better dominant-negative TNFR1 mutant than inactivation of the death domain by point mutation. The increased dominant-negative activity correlates with increased cell surface expression. The membrane proximal region is the most important region of the receptor for restricting expression.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine
Uncontrolled Keywords:	Tumour necrosis factor receptor 1; Nuclear factor-κB; Structure–function; Dominant-negative receptor; Surface expression
Publisher:	Elsevier
ISSN:	0014-5793
Last Modified:	17 Oct 2022 08:28
URI:	https://orca.cardiff.ac.uk/id/eprint/233

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