Thrombin-activated human platelets acutely generate oxidized docosahexaenoic-acid-containing phospholipids via 12-lipoxygenase

Morgan, Lloyd T., Thomas, Christopher P. ORCID: https://orcid.org/0000-0001-5840-8613, Kühn, Hartmut and O'Donnell, Valerie Bridget ORCID: https://orcid.org/0000-0003-4089-8460 2010. Thrombin-activated human platelets acutely generate oxidized docosahexaenoic-acid-containing phospholipids via 12-lipoxygenase. Biochemical Journal 431 (1) , pp. 141-148. 10.1042/BJ20100415

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Abstract

Arachidonate-containing oxidized phospholipids are acutely generated by 12-LOX (12-lipoxygenase) in agonist-activated platelets. In the present study, formation of structurally related lipids by oxidation of DHA (docosahexaenoic acid)-containing phospholipids is demonstrated using lipidomic approaches. Precursor scanning reverse-phase LC (liquid chromatography)–MS/MS (tandem MS) identified a new family of lipids that comprise phospholipid-esterified HDOHE (hydroxydocosahexaenoic acid). Two diacyl and two plasmalogen PEs (phosphatidylethanolamines) containing predominantly the 14-HDOHE positional isomer (18:0p/14-HDOHE-PE, 18:0a/14-HDOHE-PE, 16:0a/14-HDOHE-PE and 16:0p/14-HDOHE-PE) were structurally characterized using MS/MS and by comparison with biogenic standards. An involvement of 12-LOX was indicated as purified recombinant human 12-LOX also generated the 14-HDOHE isomer from DHA. Pharmacological studies using inhibitors and recombinant platelet 12-LOX indicate that they form via esterification of newly formed non-esterified HDOHE. HDOHE-PEs formed at significant rates (2–4 ng/4×107 cells) within 2–180 min of thrombin stimulation, and their formation was blocked by calcium chelation. In summary, a new family of oxidized phospholipid was identified in thrombin-activated human platelets.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > RZ Other systems of medicine
Uncontrolled Keywords:	docosahexanoic acid; lipoxygenase; mass spectrometry; phospholipid; platelet; thrombin
Publisher:	Biochemical Society
ISSN:	0264-6021
Last Modified:	19 Oct 2022 10:39
URI:	https://orca.cardiff.ac.uk/id/eprint/25131

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