Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence

Reddington, Samuel C., Rizkallah, Pierre ORCID: https://orcid.org/0000-0002-9290-0369, Watson, Peter Duncan ORCID: https://orcid.org/0000-0003-0250-7852, Pearson, Rachel, Tippmann, Eric Michael and Jones, Darran Dafydd ORCID: https://orcid.org/0000-0001-7709-3995 2013. Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence. Angewandte Chemie - International Edition 52 (23) , pp. 5974-5977. 10.1002/anie.201301490

Full text not available from this repository.

Abstract

Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Chemistry Medicine
Subjects:	Q Science > QD Chemistry
Uncontrolled Keywords:	expanded genetic code; green fluorescent protein; phenyl azide; photochemistry; protein engineering
Publisher:	Wiley-Blackwell
ISSN:	1433-7851
Funders:	BBSRC, SARTRE, EPSRC, MRC
Last Modified:	10 Jul 2024 19:15
URI:	https://orca.cardiff.ac.uk/id/eprint/48715

Citation Data

Cited 44 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item