Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles

Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571, Williams, Catrin Ffion ORCID: https://orcid.org/0000-0001-8619-2581, Vijayalakshmi, K., Kombrabail, M., White, Nicholas, Hayes, Anthony, Aon, Miguel A. and Krishnamoorthy, G. 2014. Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles. Journal of Innovative Optical Health Sciences 7 (2) , 1350041. 10.1142/S1793545813500417

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Abstract

The ability to resolve the spatio-temporal complexity of intracellular O2 distribution is the "Holy Grail" of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium–sapphire Tsunami lasers (710–1050 nm).

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Optometry and Vision Sciences
Subjects:	R Medicine > RE Ophthalmology
Uncontrolled Keywords:	Time-resolved phosphorescence; intracellular O2; two-photon excitation; pinhole shifting
Publisher:	World Scientific
ISSN:	1793-5458
Date of First Compliant Deposit:	18 February 2019
Date of Acceptance:	1 September 2013
Last Modified:	08 May 2023 18:23
URI:	https://orca.cardiff.ac.uk/id/eprint/57070

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