Lloyd, David  ORCID: https://orcid.org/0000-0002-5656-0571, Williams, Catrin Ffion ORCID: https://orcid.org/0000-0001-8619-2581, Vijayalakshmi, K., Kombrabail, M., White, Nicholas, Hayes, Anthony, Aon, Miguel A. and Krishnamoorthy, G.
2014.
Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles.
Journal of Innovative Optical Health Sciences
7
(2)
, 1350041.
10.1142/S1793545813500417
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Abstract
The ability to resolve the spatio-temporal complexity of intracellular O2 distribution is the "Holy Grail" of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium–sapphire Tsunami lasers (710–1050 nm).
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Biosciences Schools > Optometry and Vision Sciences |
| Subjects: | R Medicine > RE Ophthalmology |
| Uncontrolled Keywords: | Time-resolved phosphorescence; intracellular O2; two-photon excitation; pinhole shifting |
| Publisher: | World Scientific |
| ISSN: | 1793-5458 |
| Date of First Compliant Deposit: | 18 February 2019 |
| Date of Acceptance: | 1 September 2013 |
| Last Modified: | 08 May 2023 18:23 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/57070 |
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