The potential of IGF-1 and TGFβ1 for promoting “adult” articular cartilage repair: An in vitro study

Davies, L. C., Blain, Emma ORCID: https://orcid.org/0000-0001-8944-4254, Gilbert, Sophie Jane, Caterson, Bruce ORCID: https://orcid.org/0000-0001-6016-0661 and Duance, Victor ORCID: https://orcid.org/0000-0002-7555-2016 2008. The potential of IGF-1 and TGFβ1 for promoting “adult” articular cartilage repair: An in vitro study. Tissue Engineering Part A 14 (7) , pp. 1251-1261. 10.1089/ten.tea.2007.0211

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Abstract

Research into articular cartilage repair, a tissue unable to spontaneously regenerate once injured, has focused on the generation of a biomechanically functional repair tissue with the characteristics of hyaline cartilage. This study was undertaken to provide insight into how to improve ex vivo chondrocyte amplification, without cellular dedifferentiation for cell-based methods of cartilage repair. We investigated the effects of insulin-like growth factor 1 (IGF-1) and transforming growth factor beta 1 (TGFβ1) on cell proliferation and the de novo synthesis of sulfated glycosaminoglycans and collagen in chondrocytes isolated from skeletally mature bovine articular cartilage, whilst maintaining their chondrocytic phenotype. Here we demonstrate that mature differentiated chondrocytes respond to growth factor stimulation to promote de novo synthesis of matrix macromolecules. Additionally, chondrocytes stimulated with IGF-1 or TGFβ1 induced receptor expression. We conclude that IGF-1 and TGFβ1 in addition to autoregulatory effects have differential effects on each other when used in combination. This may be mediated by regulation of receptor expression or endogenous factors; these findings offer further options for improving strategies for repair of cartilage defects.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Dentistry
Publisher:	Thomson Reuters
ISSN:	2152-4947
Last Modified:	27 Oct 2022 09:29
URI:	https://orca.cardiff.ac.uk/id/eprint/66084

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