Theaker, Sarah M., Rius Rafael, Cristina ![]() ![]() ![]() ![]() |
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Abstract
Isolation of peptide-specific T-cell clones is highly desirable for determining the role of T-cells in human disease, as well as for the development of therapies and diagnostics. However, generation of monoclonal T-cells with the required specificity is challenging and time-consuming. Here we describe a library-based strategy for the simple parallel detection and isolation of multiple peptide-specific human T-cell clones from CD8+ or CD4+ polyclonal T-cell populations. T-cells were first amplified by CD3/CD28 microbeads in a 96U-well library format, prior to screening for desired peptide recognition. T-cells from peptide-reactive wells were then subjected to cytokine-mediated enrichment followed by single-cell cloning, with the entire process from sample to validated clone taking as little as 6 weeks. Overall, T-cell libraries represent an efficient and relatively rapid tool for the generation of peptide-specific T-cell clones, with applications shown here in infectious disease (Epstein–Barr virus, influenza A, and Ebola virus), autoimmunity (type 1 diabetes) and cancer.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Subjects: | Q Science > QR Microbiology > QR180 Immunology |
Publisher: | Elsevier |
ISSN: | 0022-1759 |
Funders: | Wellcome Trust |
Date of First Compliant Deposit: | 30 March 2016 |
Date of Acceptance: | 26 January 2016 |
Last Modified: | 15 May 2024 01:18 |
URI: | https://orca.cardiff.ac.uk/id/eprint/87394 |
Citation Data
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