An alternative spliced transcript of ADAMTS4 is present in human synovium from OA patients

Wainwright, Shane Daniel, Bondeson, Jan and Hughes, Clare Elizabeth ORCID: https://orcid.org/0000-0003-4726-5877 2006. An alternative spliced transcript of ADAMTS4 is present in human synovium from OA patients. Matrix Biology 25 (5) , pp. 317-320. 10.1016/j.matbio.2006.03.006

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Abstract

The major proteoglycan of articular cartilage aggrecan is a substrate for ADAMTS4. RT-PCR analysis of human osteoarthritic (OA) synovial co-cultures using oligonucleotide primers designed to amplify across the exon 8/9 junction of human ADAMTS4 resulted in the amplification of two products, the expected product and a smaller product missing 161 bp from the 5′ end of exon 9, the result of alternative splicing in which exon 8 joins to a cryptic 3′ splice site within exon 9. The protein produced would be identical to human ADAMTS4 up to Arg696, and would have a new C-terminal domain with no commonality with the ADAMTS4 spacer domain. Changes in the C-terminal domain of ADAMTS4 may alter its substrate specificity.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine Schools > Biosciences
Subjects:	Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords:	ADAMTS4; Synovium; Alternative splice; Osteoarthritis
Publisher:	Elsevier
ISSN:	0945-053X
Last Modified:	01 Dec 2022 10:01
URI:	https://orca.cardiff.ac.uk/id/eprint/1048

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