IL-10-conditioned dendritic cells prevent autoimmune diabetes in NOD and humanized HLA-DQ8/RIP-B7.1 mice

Tai, Ningwen, Yasuda, Hisafumi, Xiang, Yufei, Zhang, Li, Rodriguez-Pinto, Daniel, Yokono, Koichi, Sherwin, Robert, Wong, F. Susan ORCID: https://orcid.org/0000-0002-2812-8845, Nagata, Masao and Wen, Li 2011. IL-10-conditioned dendritic cells prevent autoimmune diabetes in NOD and humanized HLA-DQ8/RIP-B7.1 mice. Clinical Immunology 139 (3) , pp. 336-349. 10.1016/j.clim.2011.03.003

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Abstract

This study was to determine whether BMDCs cultured in the presence of IL-10 (G/10-DCs) could promote T cell tolerance and prevent autoimmune diabetes in two different animal models of T1D. Our results showed that G/10-DCs suppressed both insulitis and spontaneous diabetes in NOD and HLA-DQ8/RIP-B7.1 mice. The suppression was likely to be mediated by T cells, as we found that regulatory CD4+CD25+Foxp3+ cells were significantly increased in G/10-DC treated animals. In vivo, the G/10-DCs inhibited diabetogenic T cell proliferation; in vitro, they had reduced expression of costimulatory molecules and produced little IL-12/23 p40 or IL-6 but a large amount of IL-10 when compared with DCs matured in the presence of IL-4 (G/4-DC). We conclude that IL-10-treated DCs are tolerogenic and induce islet-directed immune tolerance, which was likely to be mediated by T regulatory cells. This non-antigen-specific DC-based approach offers potential for a new therapeutic intervention in T1D.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Elsevier
ISSN:	1521-6616
Date of Acceptance:	2 March 2011
Last Modified:	03 Nov 2022 09:33
URI:	https://orca.cardiff.ac.uk/id/eprint/105280

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