Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

Zaki, Athraa, Hartley, Andrew, Reddington, Samuel, Thomas, Suzanne ORCID: https://orcid.org/0000-0003-0342-3298, Watson, Peter ORCID: https://orcid.org/0000-0003-0250-7852, Hayes, Anthony, Moskalenko, Andy, Craciun, Monica, MacDonald, John ORCID: https://orcid.org/0000-0001-5504-1692, Jones, Darran Dafydd ORCID: https://orcid.org/0000-0001-7709-3995 and Elliott, Martin ORCID: https://orcid.org/0000-0002-9254-9898 2018. Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle. RSC Advances 8 , pp. 5768-5775. 10.1039/c7ra11166e

Preview

PDF - Published Version Available under License Creative Commons Attribution. Download (9MB) | Preview

Abstract

We have created modified protein variants by introducing a non-canonical amino acid p-azido-Lphenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b562 proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Physics and Astronomy
Publisher:	RSC Publishing
ISSN:	2046-2069
Funders:	EPSRC
Date of First Compliant Deposit:	27 February 2018
Date of Acceptance:	11 January 2018
Last Modified:	21 Nov 2023 08:22
URI:	https://orca.cardiff.ac.uk/id/eprint/109591

Citation Data

Cited 7 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item