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Base pair mismatch identification with DNA nanoswitch and long lifetime acridine fluorophore

McGuinness, Colin D., Keszenman-Pereyra, David, Dickinson, Paul, Campbell, Colin J., Maltman, Beatrice A., Bachmann, Till T., Ghazal, Peter ORCID: https://orcid.org/0000-0003-0035-2228 and Crain, Jason 2010. Base pair mismatch identification with DNA nanoswitch and long lifetime acridine fluorophore. Sensors and Actuators B: Chemical 148 (1) , pp. 342-346. 10.1016/j.snb.2010.04.047

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Abstract

Detection of single base pair mutations in an unlabelled nucleic acid target sequence is demonstrated using the time-resolved measurement of the Förster Resonance Energy Transfer (FRET) between a long lifetime acridine-based fluorophore and a non-fluorescent quencher molecule covalently bound to a DNA Holliday junction-based nanoswitch.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Elsevier
ISSN: 0925-4005
Date of Acceptance: 28 April 2010
Last Modified: 23 Oct 2022 14:16
URI: https://orca.cardiff.ac.uk/id/eprint/113218

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