A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets

Campbell, Colin J., Mountford, Christopher P., Stoquert, Helene C., Buck, Amy H., Dickinson, Paul, Ferapontova, Elena, Terry, Jonathan G., Beattie, John S., Walton, Anthony J., Crain, Jason, Ghazal, Peter ORCID: https://orcid.org/0000-0003-0035-2228 and Mount, Andrew R. 2009. A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets. Analyst 134 (9) , pp. 1873-1879. 10.1039/b900325h

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Abstract

DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Medicine
Publisher:	Royal Society of Chemistry
ISSN:	0003-2654
Date of Acceptance:	26 June 2009
Last Modified:	23 Oct 2022 14:16
URI:	https://orca.cardiff.ac.uk/id/eprint/113225

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