Bait-and-switch molecular recognition in nucleic acid sensors: Time-resolved fluorescence, single nucleotide polymorphism detection

Nishimura, Mira K. Y., McGuinness, Colin D., Keszenman-Pereyra, David, Dickinson, Paul, Campbell, Colin J., Bachmann, Till T., Ghazal, Peter ORCID: https://orcid.org/0000-0003-0035-2228 and Crain, Jason 2009. Bait-and-switch molecular recognition in nucleic acid sensors: Time-resolved fluorescence, single nucleotide polymorphism detection. Presented at: 2009 Symposium on Photonics and Optoelectronics, Wuhan, China, 14-16 August 2009. 2009 Symposium on Photonics and Optoelectronics. IEEE, 10.1109/SOPO.2009.5230114

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Abstract

We investigate the properties of a simple DNA-based nanodevice capable of detecting single nucleotide polymorphisms (SNPs) in unlabelled nucleic acid target sequences. Detection is achieved by a two-stage bait-and-switch process combining complementary-base hybridization and switching as molecular recognition criteria. A probe molecule is constructed from a single DNA strand designed to adopt a partial cruciform structure with a pair of exposed (unhybridized) strands. Upon target binding, a switchable cloverleaf construct (similar to a Holliday junction) is formed where the states are the open and closed junction conformations. Switching between these occurs by junction folding in the presence of divalent ions. A combination of steady-state and time-resolved fluorescence spectroscopy is used to measure Forster resonance energy transfer.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Published Online
Status:	Published
Schools:	Medicine
Publisher:	IEEE
ISBN:	9781424444120
Last Modified:	23 Oct 2022 14:16
URI:	https://orca.cardiff.ac.uk/id/eprint/113227

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