Rivellese, Felice, Mauro, Daniele, Nerviani, Alessandra, Pagani, Sara, Fossati-Jimack, Liliane, Messemaker, Tobias, Kurreeman, Fina, Toes, Rene, Rauber, Simon, Schett, Georg, Jones, Gareth, Jones, Simon A. ORCID: https://orcid.org/0000-0001-7297-9711, Rossi, Francesca, de Paulis, Amato, Marone, Gianni, El Shikh, Mohey Eldin and Humby, Frances 2018. Mast cells in early rheumatoid arthritis associate with disease severity and support B-cell autoantibody production. Annals of the Rheumatic Diseases 77 (12) , pp. 1773-1781. 10.1136/annrheumdis-2018-213418 |
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Abstract
Objectives Mast cells (MCs) are involved in the pathogenesis of rheumatoid arthritis (RA). However, their contribution remains controversial. To establish their role in RA, we analysed their presence in the synovium of treatment-naïve patients with early RA and their association and functional relationship with histological features of synovitis. Methods Synovial tissue was obtained by ultrasound-guided biopsy from treatment-naïve patients with early RA (n=99). Immune cells (CD3/CD20/CD138/CD68) and their relationship with CD117+MCs in synovial tissue were analysed by immunohistochemistry (IHC) and immunofluorescence (IF). The functional involvement of MCs in ectopic lymphoid structures (ELS) was investigated in vitro, by coculturing MCs with naïve B cells and anticitrullinated protein antibodies (ACPA)-producing B cell clones, and in vivo in interleukin-27 receptor alpha (IL27ra)-deficient and control mice during antigen-induced arthritis (AIA). Results High synovial MC counts are associated with local and systemic inflammation, autoantibody positivity and high disease activity. IHC/IF showed that MCs reside at the outer border of lymphoid aggregates. Furthermore, human MCs promote the activation and differentiation of naïve B cells and induce the production of ACPA, mainly via contact-dependent interactions. In AIA, synovial MC numbers increase in IL27ra deficient mice, in association with ELS and worse disease activity.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Publisher: | BMJ Publishing Group |
ISSN: | 0003-4967 |
Funders: | Arthritis Research UK |
Date of First Compliant Deposit: | 24 July 2018 |
Date of Acceptance: | 27 July 2018 |
Last Modified: | 06 Nov 2023 20:01 |
URI: | https://orca.cardiff.ac.uk/id/eprint/113384 |
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