Locus-specific gene expression pattern suggests a unique propagation strategy for a giant algal virus

Allen, M. J., Forster, T., Schroeder, D. C., Hall, M., Roy, D., Ghazal, P. ORCID: https://orcid.org/0000-0003-0035-2228 and Wilson, W. H. 2006. Locus-specific gene expression pattern suggests a unique propagation strategy for a giant algal virus. Journal of Virology 80 (15) , pp. 7699-7705. 10.1128/JVI.00491-06

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Abstract

Emiliania huxleyi virus strain 86 is the largest algal virus sequenced to date and is unique among the Phycodnaviridae since its genome is predicted to contain six RNA polymerase subunit genes. We have used a virus microarray to profile the temporal transcription strategy of this unusual virus during infection. There are two distinct transcription phases to the infection process. The primary phase is dominated by a group of coding sequences (CDSs) expressed by 1 h postinfection that are localized to a subregion of the genome. The CDS of the primary group have no database homologues, and each is associated with a unique promoter element. The remainder of the CDSs are expressed in a secondary phase between 2 and 4 hours postinfection. Compartmentalized transcription of the two distinctive phases is discussed. We hypothesize that immediately after infection the nucleic acid of the virus targets the host nucleus, where primary-phase genes are transcribed by host RNA polymerase which recognizes the viral promoter. Secondary-phase transcription may then be conducted in the cytoplasm

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	American Society for Microbiology
ISSN:	0022-538X
Date of Acceptance:	10 May 2006
Last Modified:	23 Oct 2022 14:20
URI:	https://orca.cardiff.ac.uk/id/eprint/113397

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