Ebrahimi, B., Dutia, Bernadette M., Roberts, Kim L., Garcia-Ramirez, Jose J., Dickinson, Paul, Stewart, James P., Ghazal, Peter  ORCID: https://orcid.org/0000-0003-0035-2228, Roy, Douglas J. and Nash, Anthony A.
2003.
Transcriptome profile of murine gammaherpesvirus-68 lytic infection.
Journal of General Virology
84
(1)
, pp. 99-109.
10.1099/vir.0.18639-0
|
Abstract
The murine gammaherpesvirus-68 genome encodes 73 protein-coding open reading frames with extensive similarities to human γ2 herpesviruses, as well as unique genes and cellular homologues. We performed transcriptome analysis of stage-specific viral RNA during permissive infection using an oligonucleotide-based microarray. Using this approach, M4, K3, ORF38, ORF50, ORF57 and ORF73 were designated as immediate-early genes based on cycloheximide treatment. The microarray analysis also identified 10 transcripts with early expression kinetics, 32 transcripts with early-late expression kinetics and 29 transcripts with late expression kinetics. The latter group consisted mainly of structural proteins, and showed high expression levels relative to other viral transcripts. Moreover, we detected all eight tRNA-like transcripts in the presence of cycloheximide and phosphonoacetic acid. Lytic infection with MHV-68 also resulted in a significant reduction in the expression of cellular transcripts included in the DNA chip. This global approach to viral transcript analysis offers a powerful system for examining molecular transitions between lytic and latent virus infections associated with disease pathogenesis.
| Item Type: | Article |
|---|---|
| Date Type: | Published Online |
| Status: | Published |
| Schools: | Medicine |
| Publisher: | Society for General Microbiology |
| ISSN: | 0022-1317 |
| Date of Acceptance: | 18 September 2002 |
| Last Modified: | 23 Oct 2022 14:22 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/113501 |
Citation Data
Cited 109 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
Altmetric
Altmetric
Cardiff University Information Services