Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism?

Gilbert, Sophie Jane, Duance, Victor Colin ORCID: https://orcid.org/0000-0002-7555-2016 and Mason, Deborah Jane ORCID: https://orcid.org/0000-0002-8666-6094 2003. Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism? Arthritis Research & Therapy 6 (1) , R46-R55. 10.1186/ar1024

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Abstract

We investigated the role of the proinflammatory cytokine TNF-?, the second messenger C2-ceramide, and protein kinase R (PKR) in bovine articular cartilage degradation. Bovine articular cartilage explants were stimulated with C2-ceramide or TNF-? for 24 hours. To inhibit the activation of PKR, 2-aminopurine was added to duplicate cultures. Matrix metalloproteinase (MMP) expression and activation in the medium were analysed by gelatin zymography, proteoglycan release by the dimethylmethylene blue assay, and cell viability by the Cytotox 96

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Uncontrolled Keywords:	articular cartilag; ceramide; matrix metalloproteinase; PKR; TNF-?
Additional Information:	Publisher’s copyright requirements: see http://www.biomedcentral.com/info/about/license
ISSN:	1478-6354
Last Modified:	13 May 2023 00:36
URI:	https://orca.cardiff.ac.uk/id/eprint/1170

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