Impact of black raspberries on the normal and malignant Apc deficient murine gut microbiome

May, Stephanie, McDermott, Grace, Marchesi, Julian ORCID: https://orcid.org/0000-0002-7994-5239 and Parry, Lee ORCID: https://orcid.org/0000-0002-4467-9196 2020. Impact of black raspberries on the normal and malignant Apc deficient murine gut microbiome. Journal of Berry Research 10 (1) , pp. 61-76. 10.3233/JBR-180372

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Abstract

BACKGROUND: In animals and humans black raspberries (BRBs) have chemo-preventative effects against Wnt driven colorectal cancer (CRC). While BRBs have made it into clinical trials, the exact mechanisms of BRB action remain unclear. Potentially the chemo-preventative properties are linked to their impact on the gut microbiome, as diet is known to influence the microbial diversity of the gut and plays a key role in regulating intestinal homeostasis and the aetiology of CRC. OBJECTIVE: To determine the impact of a BRB diet on themicrobial biodiversityof thewild-type and malignant mouse intestine. METHODS: Adult mice in which Wnt driven tumourigenesis could be initiated by conditional deletion of Apc in the intestinal stem cell (Lgr5CreERT2) were administered a 10% BRB diet. Total DNA from faecal pellets pre- and post-BRB exposure was used for longitudinal metataxonomic analysis of the V1 to V3 regions of the 16S rRNA gene. RESULTS: Individually BRB intervention and Apc loss alter the microbial community of the gut. In combination, the microbiome changes observed in the Apc deficient intestine are attenuated upon administration of a BRB diet. CONCLUSIONS: Our results suggest that BRB intervention may protectively regulate the gut microbiota in the healthy and malignant intestine.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Publisher:	IOS Press
ISSN:	1878-5093
Funders:	Cancer Research UK
Date of First Compliant Deposit:	3 December 2018
Date of Acceptance:	30 November 2018
Last Modified:	05 May 2023 07:08
URI:	https://orca.cardiff.ac.uk/id/eprint/117298

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