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Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions

Nobre, Luis V., Nightingale, Katie, Ravenhill, Benjamin J., Antrobus, Robin, Soday, Lior, Nichols, Jenna, Davies, James ORCID:, Seirafian, Sepehr, Wang, Eddie C.Y. ORCID:, Davison, Andrew J., Wilkinson, Gavin W.G., Stanton, Richard J. ORCID:, Huttlin, Edward L. and Weekes, Michael P. 2019. Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions. eLife 8 , e49894. 10.7554/eLife.49894

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Human cytomegalovirus (HCMV) extensively modulates host cells, downregulating >900 human proteins during viral replication and degrading ≥133 proteins shortly after infection. The mechanism of degradation of most host proteins remains unresolved, and the functions of many viral proteins are incompletely characterised. We performed a mass spectrometry-based interactome analysis of 169 tagged, stably-expressed canonical strain Merlin HCMV proteins, and two non-canonical HCMV proteins, in infected cells. This identified a network of >3,400 virus-host and >150 virus-virus protein interactions, providing insights into functions for multiple viral genes. Domain analysis predicted binding of the viral UL25 protein to SH3 domains of NCK Adaptor Protein-1. Viral interacting proteins were identified for 31/133 degraded host targets. Finally, the uncharacterised, non-canonical ORFL147C protein was found to interact with elements of the mRNA splicing machinery, and a mutational study suggested its importance in viral replication. The interactome data will be important for future studies of herpesvirus infection.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Publisher: eLife Sciences Publications
ISSN: 2050-084X
Funders: Medical Research Council, Wellcome Trust
Date of First Compliant Deposit: 7 January 2020
Date of Acceptance: 24 December 2019
Last Modified: 07 Nov 2023 23:42

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