Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

The identity of human tissue-emigrant CD8+ T cells

Buggert, Marcus, Vella, Laura A., Nguyen, Son, Wu, Vincent H., Chen, Zeyu, Sekine, Takuya, Perez-Potti, André, Maldini, Colby R., Manne, Sasikanth, Darko, Samuel, Ransier, Amy, Kuri-Cervantes, Leticia, Japp, Alberto Sada, Brody, Irene Bukh, Ivarsson, Martin A., Gorin, Jean-Baptiste, Rivera-Ballesteros, Olga, Hertwig, Laura, Antel, Jack P., Johnson, Matthew E., Okoye, Afam, Picker, Louis, Vahedi, Golnaz, Sparrelid, Ernesto, Llewellyn-Lacey, Sian, Gostick, Emma, Sandberg, Johan K., Björkström, Niklas, Bar-Or, Amit, Dori, Yoav, Naji, Ali, Canaday, David H., Laufer, Terri M., Wells, Andrew D., Price, David A., Frank, Ian, Douek, Daniel C., Wherry, E. John, Itkin, Maxim G. and Betts, Michael R. 2020. The identity of human tissue-emigrant CD8+ T cells. Cell 183 (7) , pp. 1946-1961. 10.1016/j.cell.2020.11.019

[thumbnail of Buggert et al_revised manuscript_Cell-MB_DAP.pdf]
PDF - Accepted Post-Print Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (17MB) | Preview


Lymphocyte migration is essential for adaptive immune surveillance. However, our current understanding of this process is rudimentary, because most human studies have been restricted to immunological analyses of blood and various tissues. To address this knowledge gap, we used an integrated approach to characterize tissue-emigrant lineages in thoracic duct lymph (TDL). The most prevalent immune cells in human and non-human primate efferent lymph were T cells. Cytolytic CD8 + T cell subsets with effector-like epigenetic and transcriptional signatures were clonotypically skewed and selectively confined to the intravascular circulation, whereas non-cytolytic CD8 + T cell subsets with stem-like epigenetic and transcriptional signatures predominated in tissues and TDL. Moreover, these anatomically distinct gene expression profiles were recapitulated within individual clonotypes, suggesting parallel differentiation programs independent of the expressed antigen receptor. Our collective dataset provides an atlas of the migratory immune system and defines the nature of tissue-emigrant CD8 + T cells that recirculate via TDL.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Elsevier (Cell Press)
ISSN: 0092-8674
Date of First Compliant Deposit: 14 January 2021
Date of Acceptance: 12 November 2020
Last Modified: 14 May 2022 08:20

Citation Data

Cited 19 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics