Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

JMJD6 promotes self-renewal and regenerative capacity of hematopoietic stem cells

Lawson, Hannah, Sepulveda, Catarina, van de Latemaat, Louie N., Durko, Jozef, Barile, Melania, Tavosanis, Andrea, Georges, Elise, Shmakova, Alena, Timms, Penny, Carter, Roderick N., Allen, Lewis, Campos, Joana, Vukovic, Milica, Guitart, Amelie V., Giles, Peter ORCID:, O'Shea, Marie, Vernimmen, Douglas, Morton, Nicholas M., Rodrigues, Neil P. ORCID:, Gottgens, Berthold, Schofield, Christopher J., Lengeling, Andreas, O'Carroll, Donal and Kranc, Kamil R. 2021. JMJD6 promotes self-renewal and regenerative capacity of hematopoietic stem cells. Blood Advances 5 (3) , pp. 889-899. 10.1182/bloodadvances.2020002702

[thumbnail of advancesadv2020002702.pdf] PDF - Published Version
Download (1MB)


Lifelong multilineage hematopoiesis critically depends on rare hematopoietic stem cells (HSCs) that reside in the hypoxic bone marrow microenvironment. Although the role of the canonical oxygen sensor hypoxia-inducible factor prolyl hydroxylase has been investigated extensively in hematopoiesis, the functional significance of other members of the 2-oxoglutarate (2-OG)-dependent protein hydroxylase family of enzymes remains poorly defined in HSC biology and multilineage hematopoiesis. Here, by using hematopoietic-specific conditional gene deletion, we reveal that the 2-OG–dependent protein hydroxylase JMJD6 is essential for short- and long-term maintenance of the HSC pool and multilineage hematopoiesis. Additionally, upon hematopoietic injury, Jmjd6-deficient HSCs display a striking failure to expand and regenerate the hematopoietic system. Moreover, HSCs lacking Jmjd6 lose multilineage reconstitution potential and self-renewal capacity upon serial transplantation. At the molecular level, we found that JMJD6 functions to repress multiple processes whose downregulation is essential for HSC integrity, including mitochondrial oxidative phosphorylation (OXPHOS), protein synthesis, p53 stabilization, cell cycle checkpoint progression, and mTORC1 signaling. Indeed, Jmjd6-deficient primitive hematopoietic cells display elevated basal and maximal mitochondrial respiration rates and increased reactive oxygen species (ROS), prerequisites for HSC failure. Notably, an antioxidant, N-acetyl-l-cysteine, rescued HSC and lymphoid progenitor cell depletion, indicating a causal impact of OXPHOS-mediated ROS generation upon Jmjd6 deletion. Thus, JMJD6 promotes HSC maintenance and multilineage differentiation potential by suppressing fundamental pathways whose activation is detrimental for HSC function.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Publisher: American Society of Hematology: Blood Advances
ISSN: 2473-9529
Date of First Compliant Deposit: 10 February 2021
Date of Acceptance: 1 December 2020
Last Modified: 05 May 2023 02:31

Citation Data

Cited 3 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics