Cai, Shuo, Sun, Zhiwei, Sun, Ping-Hui, Gao, Xiangyu, Ji, Ke, Tian, Xiuyun, Ji, Jiafu, Hao, Chunyi, Soliman, Faris, Liu, Chang, Al-Sarireh, Bilal, Griffiths, Paul, Hiscox, Stephen ![]() ![]() ![]() |
Preview |
PDF
- Published Version
Download (1MB) | Preview |
Abstract
Kidins220 is a transmembrane scaffold protein involved in several types of cancer. The aim of the present study was to examine the role of Kidins220 in tumorigenesis and disease progression of pancreatic cancer. The relevant signalling pathways including EGFR, EMT, and MMP were also investigated. The expression of Kidins220 was examined at the transcript and protein level. The Kidins220 knockdown cell model was established and its influence on cellular functions was determined. Involvement of Kidins220 in tumorigenesis and metastasis was examined in CD1 mice, respectively. The results showed that, reduced Kidin220 expression was associated with tumorigenesis, metastasis, and overall survival of pancreatic cancer. Knockdown of Kidins220 promoted proliferation, colony formation and tumorigenic capacity of pancreatic cancer cells in vitro and in vivo, respectively. Kidins220 regulated pancreatic cancer cell migration through the EGFR/AKT/ERK signalling pathway. Furthermore, enhanced EMT was observed in the pancreatic cancer cell lines with the knockdown of Kidins220, underlying EGFR regulation. Kidins220 also affected cell invasion via MMP1. A reduced expression of Kidins220 was observed in pancreatic cancer, which is associated with disease progression, distant metastasis and poor prognosis. The loss of Kidins220 in pancreatic cancer may contribute to disease progression through the upregulation of EGFR and downstream signalling.
Item Type: | Article |
---|---|
Date Type: | Publication |
Status: | Published |
Schools: | Medicine Pharmacy |
Publisher: | Spandidos Publications |
ISSN: | 1019-6439 |
Date of First Compliant Deposit: | 6 May 2021 |
Date of Acceptance: | 13 April 2021 |
Last Modified: | 16 Aug 2024 14:31 |
URI: | https://orca.cardiff.ac.uk/id/eprint/140957 |
Actions (repository staff only)
![]() |
Edit Item |