Chaprov, Kirill D., Lysikova, Ekaterina A., Teterina, Ekaterina V. and Buchman, Vladimir L.  ORCID: https://orcid.org/0000-0002-7631-8352
2021.
Kinetics of alpha-synuclein depletion in three brain regions following conditional pan-neuronal inactivation of the encoding gene (Snca) by tamoxifen-induced Cre-recombination in adult mice.
Transgenic Research
30
, pp. 867-873.
10.1007/s11248-021-00286-3
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Abstract
Conditional pan-neuronal inactivation of the Snca gene in 2-month old male and female mice causes dramatic decrease in the level of the encoded protein, alpha-synuclein, in three studied brain regions, namely cerebral cortex, midbrain and striatum, 12 weeks after the last injection of tamoxifen. Kinetics of alpha-synuclein depletion is different in these brain regions with a longer lag period in the cerebral cortex where this protein is normally most abundant. Our results suggest that efficient post-developmental pan-neuronal knockout of alpha-synuclein in adult, i.e. 5- to 6-month old, animals, could be achieved by tamoxifen treatment of 2-month old mice carrying loxP-flanked Snca gene and expressing inducible Cre-ERT2 recombinase under control of the promoter of neuron-specific enolase (NSE) gene.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Biosciences |
| Additional Information: | This article is licensed under a Creative Commons Attribution 4.0 International License |
| Publisher: | Springer |
| ISSN: | 0962-8819 |
| Date of First Compliant Deposit: | 23 September 2021 |
| Date of Acceptance: | 14 September 2021 |
| Last Modified: | 18 May 2023 05:50 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/144381 |
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