A time-kill assay study on the synergistic bactericidal activity of pomegranate rind extract and Zn (II) against Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa

Alrashidi, Amal, Jafar, Mohammed, Higgins, Niamh, Mulligan, Ciara, Varricchio, Carmine ORCID: https://orcid.org/0000-0002-1673-4768, Moseley, Ryan ORCID: https://orcid.org/0000-0002-2812-6735, Celiksoy, Vildan, Houston, David MJ and Heard, Charles M ORCID: https://orcid.org/0000-0001-9703-9777 2021. A time-kill assay study on the synergistic bactericidal activity of pomegranate rind extract and Zn (II) against Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa. Biomolecules 11 (12) , 1889. 10.3390/biom11121889

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Abstract

There is a need for new antimicrobial systems due to increased global resistance to current antimicrobials. Pomegranate rind extract (PRE) and Zn (II) ions both possess a level of antimicrobial activity and work has previously shown that PRE/Zn (II) in combination possesses synergistic activity against Herpes simplex virus and Micrococcus luteus. Here, we determined whether such synergistic activity extended to other, more pathogenic, bacteria. Reference strains of methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa were cultured and subjected to challenge by PRE, Zn (II), or PRE + Zn (II), in time-kill assays. Data were obtained independently by two researchers using different PRE preparations. Statistically significant synergistic activity for PRE + Zn (II) was shown for all four bacterial strains tested compared to untreated controls, although the extent of efficacy and timescales varied. Zn (II) exerted activity and at 1 h, it was not possible to distinguish with PRE + Zn (II) combination treatment in all cases. PRE alone showed low activity against all four bacteria. Reproducible synergistic bactericidal activity involving PRE and Zn (II) has been confirmed. Potential mechanisms are discussed. The development of a therapeutic system that possesses demonstrable antimicrobial activity is supported which lends itself particularly to topical delivery applications, for example MRSA infections.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy Dentistry
Publisher:	MDPI
ISSN:	2218-273X
Date of First Compliant Deposit:	21 December 2021
Date of Acceptance:	13 December 2021
Last Modified:	06 Jan 2024 02:30
URI:	https://orca.cardiff.ac.uk/id/eprint/146264

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