Metabolic regulation by prostaglandin E2 impairs lung group 2 innate lymphoid cell responses

Robb, Calum T., Zhou, You ORCID: https://orcid.org/0000-0002-1743-1291, Felton, Jennifer M., Zhang, Birong, Goepp, Marie, Jheeta, Privjyot, Smyth, Danielle J., Duffin, Rodger, Vermeren, Sonja, Breyer, Richard M., Narumiya, Shuh, McSorley, Henry J., Maizels, Rick M., Schwarze, Jürgen K. J., Rossi, Adriano G. and Yao, Chengcan 2023. Metabolic regulation by prostaglandin E2 impairs lung group 2 innate lymphoid cell responses. Allergy 78 (3) , pp. 714-730. 10.1111/all.15541

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Abstract

Background: Group 2 innate lymphoid cells (ILC2s) play a critical role in asthma pathogenesis. Non‐steroidal anti‐inflammatory drug (NSAID)‐exacerbated respiratory disease (NERD) is associated with reduced signaling via EP2, a receptor for prostaglandin E2 (PGE2). However, the respective roles for the PGE2 receptors EP2 and EP4 (both share same downstream signaling) in the regulation of lung ILC2 responses has yet been deciphered. Methods: The roles of PGE2 receptors EP2 and EP4 on ILC2‐mediated lung inflammation were investigated using genetically modified mouse lines and pharmacological approaches in IL‐33‐induced lung allergy model. The effects of PGE2 receptors and downstream signals on ILC2 metabolic activation and effector function were examined using in vitro cell cultures. Results: Deficiency of EP2 rather than EP4 augments IL‐33‐induced mouse lung ILC2 responses and eosinophilic inflammation in vivo. In contrast, exogenous agonism of EP4 and EP2 or inhibition of phosphodiesterase markedly restricts IL‐33‐induced lung ILC2 responses. Mechanistically, PGE2 directly suppresses IL‐33‐dependent ILC2 activation through the EP2/EP4‐cAMP pathway, which downregulates STAT5 and MYC pathway gene expression and ILC2 energy metabolism. Blocking glycolysis diminishes IL‐33‐dependent ILC2 responses in mice where endogenous PG synthesis or EP2 signaling is blocked but not in mice with intact PGE2‐EP2 signaling. Conclusion: We have defined a mechanism for optimal suppression of mouse lung ILC2 responses by endogenous PGE2‐EP2 signaling which underpins the clinical findings of defective EP2 signaling in patients with NERD. Our findings also indicate that exogenously targeting the PGE2‐EP4‐cAMP and energy metabolic pathways may provide novel opportunities for treating the ILC2‐initiated lung inflammation in asthma and NERD.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Additional Information:	License information from Publisher: LICENSE 1: URL: http://creativecommons.org/licenses/by/4.0/
Publisher:	Wiley
ISSN:	0105-4538
Date of First Compliant Deposit:	17 October 2022
Date of Acceptance:	18 September 2022
Last Modified:	05 May 2023 09:59
URI:	https://orca.cardiff.ac.uk/id/eprint/153455

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