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The crystal structure of Bacillus thuringiensis Tpp80Aa1 and its interaction with galactose-containing glycolipids

Best, Hannah L., Williamson, Lainey J., Lipka-Lloyd, Magdalena, Waller-Evans, Helen ORCID: https://orcid.org/0000-0003-4133-6064, Lloyd-Evans, Emyr ORCID: https://orcid.org/0000-0002-3626-1611, Rizkallah, Pierre J. ORCID: https://orcid.org/0000-0002-9290-0369 and Berry, Colin ORCID: https://orcid.org/0000-0002-9943-548X 2022. The crystal structure of Bacillus thuringiensis Tpp80Aa1 and its interaction with galactose-containing glycolipids. Toxins 14 (12) , 863. 10.3390/toxins14120863

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Abstract

Tpp80Aa1 from Bacillus thuringiensis is a Toxin_10 family protein (Tpp) with reported action against Culex mosquitoes. Here, we demonstrate an expanded target range, showing Tpp80Aa1 is also active against the larvae of Anopheles gambiae and Aedes aegypti mosquitoes. We report the first crystal structure of Tpp80Aa1 at a resolution of 1.8 Å, which shows Tpp80Aa1 consists of two domains: an N-terminal β-trefoil domain resembling a ricin B lectin and a C-terminal putative pore-forming domain sharing structural similarity with the aerolysin family. Similar to other Tpp family members, we observe Tpp80Aa1 binds to the mosquito midgut, specifically the posterior midgut and the gastric caecum. We also identify that Tpp80Aa1 can interact with galactose-containing glycolipids and galactose, and this interaction is critical for exerting full insecticidal action against mosquito target cell lines.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Biosciences
Pharmacy
Publisher: MDPI
ISSN: 2072-6651
Funders: BBSRC
Date of First Compliant Deposit: 8 December 2022
Date of Acceptance: 24 November 2022
Last Modified: 15 Jun 2023 17:05
URI: https://orca.cardiff.ac.uk/id/eprint/154751

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