Obstructive sleep apnea affects lacrimal gland function

Wang, Shaopan, He, Xin, Li, Qingmin, Zhang, Yuhan, Hu, Jiaoyue, Zong, Rongrong, Zhuang, Jingyi, Quantock, Andrew J. ORCID: https://orcid.org/0000-0002-2484-3120, Gao, Yingying, Li, Wei and Liu, Zuguo 2022. Obstructive sleep apnea affects lacrimal gland function. Investigative Ophthalmology & Visual Science 63 (3) , 3. 10.1167/iovs.63.3.3

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Abstract

Purpose: To determine the effect of obstructive sleep apnea syndrome (OSA) on lacrimal gland function and its mechanism. Methods: Male mice aged seven to eight weeks were housed in cages with cyclic intermittent hypoxia to mimic OSA, and the control group was kept in a normal environment. Slit-lamp observation, fluorescein staining, and corneal sensitivity detection are used to assess cornea changes. Tear secretion was detected by phenol red cotton thread, and the pathological changes of lacrimal gland were observed by hematoxylin and eosin staining, oil red O staining, cholesterol and triglyceride kits, immunofluorescence staining, immunohistochemical staining, real-time polymerase chain reaction, transmission electron microscopy, and Western blot. Results: Studies revealed a decreased tear secretion, corneal epithelial defects and corneal hypersensitivity. Myoepithelial cell damage, abnormal lipid accumulation, reduced cell proliferation, increased apoptosis and inflammatory cell infiltration in the lacrimal gland were also seen. Hifα and NF-κB signaling pathways, moreover, were activated, while Pparα was downregulated, in the lacrimal glands of OSA mice. Fenofibrate treatment significantly alleviated pathological changes of the lacrimal gland induced by OSA. Conclusion: OSA disturbs the Hifα/Pparα/NF-κB signaling axis, which affects lacrimal gland structure and function and induces dry eye.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Publisher:	Association for Research in Vision and Ophthalmology
ISSN:	1552-5783
Date of First Compliant Deposit:	21 February 2023
Date of Acceptance:	12 February 2022
Last Modified:	03 May 2023 21:43
URI:	https://orca.cardiff.ac.uk/id/eprint/157165

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