Fosang, A J, Last, K, Neame, P J, Murphy, G, Knauper, Vera  ORCID: https://orcid.org/0000-0002-3965-9924, Tschesche, H, Hughes, C E, Caterson, B and Hardingham, T E
1994.
Neutrophil collagenase (MMP-8) cleaves at the aggrecanase site E373-A(374) in the interglobular domain of cartilage aggrecan.
Biochemical Journal
304
(2)
, pp. 347-351.
10.1042/bj3040347
|
Abstract
Native and recombinant neutrophil collagenase (MMP-8) was shown to cleave at the E373-A374 ‘aggrecanase’ site in the interglobular domain of aggrecan. The time course of digestion in vitro showed that MMP-8 cleaved initially at N341-F342, the predominant metalloproteinase site, before cleaving at the E373-A374 site. A synthetic peptide, IPENFFG, inhibited cleavage at E373-A374 but not N341-F342 in vitro, indicating that the E373-A374 sequence was a less preferred site for MMP-8 cleavage than N341-F342. IPENFFG also inhibited release of A374 RGSVI fragments from cartilage in explant culture, suggesting that a metalloproteinase cleaved at the aggrecanase site in situ. The possibility remains that ‘aggrecanase’ may be a metalloproteinase in cartilage.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Dentistry |
| Publisher: | Portland Press |
| ISSN: | 0264-6021 |
| Last Modified: | 15 Sep 2023 09:56 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/161597 |
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