Partial amino acid sequence of human PMN leukocyte procollagenase

Knauper, V ORCID: https://orcid.org/0000-0002-3965-9924, Kramer, S, Reinke, H and Tschesche, H 1990. Partial amino acid sequence of human PMN leukocyte procollagenase. Biological Chemistry Hoppe-Seyler 371 (5 SUPP) , pp. 295-304. 10.1515/bchm3.1990.371.2.733

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Abstract

Human PMN leukocyte procollagenase was purified to apparent homogeneity using ion exchange chromatography on Q-Sepharose Fast Flow, affinity chromatography on Zinc Chelate Sepharose and on Orange Sepharose and was finally purified by gelpermeation chromatography on Sephacryl S-300. It was shown by SDS-PAGE under reducing and non-reducing conditions that PMNL procollagenase consists of a single polypeptide chain with an apparent M(r) 85000. Amino acid sequence information was obtained from the proenzyme and fragements by Edman degradation and showed that PMNL procollagenase is homologous to the fibroblast enzyme. Thus PMNL procollagenase is the product of an independent gene.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	De Gruyter
ISSN:	1431-6730
Last Modified:	31 Aug 2023 16:00
URI:	https://orca.cardiff.ac.uk/id/eprint/161605

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