Antagonistic roles of human platelet integrin αIIbβ3 and chemokines in regulating neutrophil activation and fate on arterial thrombi under flow

Schönichen, Claudia, Montague, Samantha J., Brouns, Sanne L.N., Burston, James J., Cosemans, Judith M.E.M., Jurk, Kerstin, Kehrel, Beate E., Koenen, Rory R., Ní Áinle, Fionnuala, O'Donnell, Valerie B. ORCID: https://orcid.org/0000-0003-4089-8460, Soehnlein, Oliver, Watson, Steve P., Kuijpers, Marijke J.E., Heemskerk, Johan W.M. and Nagy, Magdolna 2023. Antagonistic roles of human platelet integrin αIIbβ3 and chemokines in regulating neutrophil activation and fate on arterial thrombi under flow. Arteriosclerosis, Thrombosis, and Vascular Biology 43 , pp. 1700-1712. 10.1161/ATVBAHA.122.318767

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Abstract

Background: Platelets and neutrophils are the first blood cells accumulating at sites of arterial thrombus formation, and both cell types contribute to the pathology of thrombotic events. We aimed to identify key interaction mechanisms between these cells using microfluidic approaches. Methods: Whole-blood perfusion was performed over a collagen surface at arterial shear rate. Platelet and leukocyte (in majority neutrophil) activation were microscopically visualized using fluorescent markers. The contributions of platelet-adhesive receptors (integrin, P-selectin, CD40L) and chemokines were studied by using inhibitors or antibodies and using blood from patients with Glanzmann thrombasthenia lacking platelet-expressed αIIbβ3. Results: We observed (1) an unknown role of activated platelet integrin αIIbß3 preventing leukocyte adhesion, which was overcome by short-term flow disturbance provoking massive adhesion; (2) that platelet-expressed CD40L controls the crawling pattern and thrombus fidelity of the cells on a thrombus; (3) that continued secretion of platelet substances promotes activation of identified neutrophils, as assessed by (fMLP induced) [Ca2+]i rises and antigen expression; (4) and that platelet-released chemokines activate the adhered cells in the order of CXCL7>CCL5>CXCL4. Furthermore, postsilencing of the platelets in a thrombus suppressed the leukocyte activation. However, the leukocytes on thrombi did no more than limitedly form neutrophil extracellular traps, unless stimulated with phorbol ester of lipopolysaccharide. Conclusions: Together, these findings reveal a multifaceted regulation of adhesion and activation of neutrophils by platelets in a thrombus, with a balanced role of several platelet-adhesive receptors and a promoting role of platelet-released substances. This multivalent nature of neutrophil-thrombus interactions offers novel prospects for pharmacological intervention.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	American Heart Association
ISSN:	1079-5642
Date of First Compliant Deposit:	18 August 2023
Date of Acceptance:	14 June 2023
Last Modified:	30 Sep 2023 22:31
URI:	https://orca.cardiff.ac.uk/id/eprint/161907

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