CD62L (L-Selectin) down-regulation does not affect memory T cell distribution but failure to shed compromises anti-viral immunity

Richards, Hannah Catharine, Longhi, Maria Paula, Wright, Kate Louise, Gallimore, Awen Myfanwy ORCID: https://orcid.org/0000-0001-6675-7004 and Ager, Ann 2008. CD62L (L-Selectin) down-regulation does not affect memory T cell distribution but failure to shed compromises anti-viral immunity. Journal of Immunology 180 (1) , pp. 198-206.

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Abstract

The down-regulation of CD62L that accompanies T lymphocyte activation is thought to redirect cells away from lymph nodes to sites of infection. In this study, CD62L was maintained on Ag-activated T cells and their distribution, and ability to clear pathogen from peripheral sites determined. CD62L was down-regulated on Ag-specific CD8 T cells in lungs of C57BL/6 mice but maintained in CD62L transgenic mice at day 8 after influenza infection. However, the numbers of influenza-specific CD8 T cells recruited were similar in CD62L transgenic and C57BL/6 mice. Memory CD8 T cell numbers in the lungs and noninvolved organs 100 days after primary infection were similar in CD62L transgenic and C57BL/6 mice, despite differing CD62L expression. Transgenic mice expressing wild-type CD62L cleared a recombinant vaccinia virus expressing an influenza-derived CD8 T cell epitope as efficiently as C57BL/6 mice. However, transgenic mice expressing a protease resistant mutant of CD62L showed significantly delayed viral clearance, despite normal CTL generation and the presence of CD107a and IFN-γ expressing influenza-specific CD8 T cells. These results demonstrate that CD62L down-regulation is not required for CD8 memory cells to home to sites of infection. However, their ability to clear virus is significantly compromised if CD62L shedding is abrogated.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine > R Medicine (General)
Publisher:	American Association of Immunologists
ISSN:	1550-6606
Last Modified:	18 Oct 2022 14:02
URI:	https://orca.cardiff.ac.uk/id/eprint/16257

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