Preparation screening, production optimization and characterization of exopolysaccharides produced by Lactobacillus sanfranciscensis Ls-1001 isolated from Chinese traditional sourdough

Zhang, Guohua, Zhang, Weizhen, Sun, Lijun, Sadiq, Faizan Ahmed ORCID: https://orcid.org/0000-0003-1596-4155, Yang, Yukun, Gao, Jie and Sang, Yaxin 2019. Preparation screening, production optimization and characterization of exopolysaccharides produced by Lactobacillus sanfranciscensis Ls-1001 isolated from Chinese traditional sourdough. International Journal of Biological Macromolecules 139 , pp. 1295-1303. 10.1016/j.ijbiomac.2019.08.077

Full text not available from this repository.

Abstract

The present study focused on preparation screening, production optimization and characterization of exopolysaccharides (EPS) produced by Lactobacillus sanfranciscensis isolated from a Chinese traditional sourdough sample. In addition, in vitro antioxidant activity of pure EPS was determined. A total of 51 strains of L. sanfranciscensis were screened, and L. sanfranciscensis Ls-1001 was proved as the most abundant crude EPS producing strain, which was selected for further EPS optimization and characterization. The optimized conditions resulted in around 31% increase in EPS production (final yield: 249.30 mg/L) and included: maltose 25 g/L, yeast peptone 10.24 g/L and fresh yeast extractives (FYE) 12.92 mL/L. HPLC analysis showed that pure EPS contained only glucose monomers, indicating that it was a kind of homopolysaccharides. FTIR spectroscopy revealed the presence of hydroxyl and carbonyl groups. NMR analysis confirmed that EPS contained α-glycosidic bond and pyranose residue. The EPS showed strong in vitro antioxidant activity.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Dentistry
Publisher:	Elsevier
ISSN:	0141-8130
Last Modified:	03 May 2024 01:29
URI:	https://orca.cardiff.ac.uk/id/eprint/168410

Actions (repository staff only)

Edit Item