Acoustic concentration of bioparticles

Dumcius, Povilas 2024. Acoustic concentration of bioparticles. PhD Thesis, Cardiff University. Item availability restricted.

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Abstract

This dissertation delivers substantial contributions to the domain of acoustofluidics, with a distinct emphasis on formulating innovative fabrication procedures, studying wave modes, and advancing particle manipulation techniques. The research presents the Dynamic Acoustofluidic System (DAS), constructed by exploiting Flexible Printed Circuit Board (FPCB) Interdigitated Electrodes (IDEs). This approach revolutionizes the fabrication of Surface Acoustic Wave (SAW)-based acoustofluidic systems, enhancing cost efficiency, accessibility, and the potential for rapid prototyping. The Dynamic Acoustofluidic System (DAS) displays exceptional versatility by permitting the positioning of the IDT transducer at any desired angle relative to the piezoelectric substrate. This capability reveals wave modes beyond the recognized Rayleigh surface acoustic waves (SAWs). By strategically manipulating frequencies and exercising precise control over the positioning of the IDT, unprecedented control over streaming patterns has been accomplished, pushing past the limitations of nanoparticle manipulation as delineated in the existing literature. Rigorous experimental investigations into liquid acoustic interactions form the core of this thesis, providing a detailed understanding of these complex processes. The use of FEM (Finite Element Method) simulations is primarily to hypothesize and visualize the underlying phenomena observed experimentally. These experimental insights have led to the development of the Acoustic Concentration of Extracellular Vesicles (ACEV) device, representing a significant advancement in rapid nanoparticle concentration techniques. The ACEV device, utilizing dual-wave mode, can efficiently concentrate nanoparticles as small as 20 nm from sample volumes of 50 µL within minutes. Importantly, this method maintains the integrity of extracellular vesicles (EVs) during the concentration process, ensuring the safe extraction of enriched particles while minimizing unwanted protein aggregates. Further refinement and automation of this concentration process hold great potential for enhanced performance and increased throughput.

Item Type:	Thesis (PhD)
Date Type:	Completion
Status:	Unpublished
Schools:	Engineering
Uncontrolled Keywords:	1). Acoustofluidics 2). Concentration 3). Extracellular Vesicles (EVs) 4). Surface Acoustic Waves 5). Nanoparticles 6). Dual-Wave Mode
Date of First Compliant Deposit:	12 June 2024
Last Modified:	13 Jun 2024 08:44
URI:	https://orca.cardiff.ac.uk/id/eprint/169752

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