Madden, Tracie-Ann ![]() ![]() |
Abstract
Selective oestrogen receptor downregulators (SERDs) are a class of highly effective steroidal antitumour agents that reduce cellular levels of the oestrogen receptor (ER). In this study, we compared the efficacy by which three novel molecular approaches: (1) antisense oligonucleotides; (2) antisense RNA; and (3) dominant negative mutants are able to act as SERDs. Using transient and, where appropriate, stable gene transfection experiments we found that constitutive overexpression of ER antisense RNA and a hormone-binding domain compromised dominant-negative ER mutant (DNER-1), were most effective at downregulating ER expression and/or activity in vitro.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Pharmacy Medicine |
Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RM Therapeutics. Pharmacology |
Uncontrolled Keywords: | Breast cancer; Oestrogen receptor; Antisense; Dominant-negative; Gene inhibition |
Publisher: | Elsevier |
ISSN: | 0014-2964 |
Last Modified: | 18 Oct 2022 14:20 |
URI: | https://orca.cardiff.ac.uk/id/eprint/17173 |
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