A method for analysis of oxidized phospholipids from biological samples using mass spectrometry

Ghorasaini, Mohan, Costa, Daniela ORCID: https://orcid.org/0000-0001-8821-5898, Tyrrell, Victoria J., Protty, Majd, Giera, Martin and O'Donnell, Valerie B. ORCID: https://orcid.org/0000-0003-4089-8460 2025. A method for analysis of oxidized phospholipids from biological samples using mass spectrometry. Giera, M. and Sánchez-López, E., eds. Methods in Molecular Biology, Vol. 2855. New York, NY, USA: Springer, pp. 155-169. (10.1007/978-1-0716-4116-3_10)

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Abstract

Oxidized phospholipids (oxPLs) are generated during innate immunity and inflammation, where they play a variety of biological roles, including regulation of autoimmunity and coagulation. Some are generated by enzymatic reactions, leading to stereo- and regiospecificity, while many others can be formed through nonenzymatic oxidation and truncation and can be used as biomarkers of oxidative stress. Mass spectrometry methods have been developed over many years for oxPL analysis, which can provide robust estimations of molecular species and amounts, where standards are available. Here we present a method used for the analysis of enzymatically-generated oxPL (eoxPL), which allows quantification of mono-hydroxy oxylipin-containing species. We also show profiling of many other partially characterized structures in tissue samples and provide typical chromatograms obtained.

Item Type:	Book Section
Status:	Published
Schools:	Medicine
Publisher:	Springer
ISBN:	9781071641156
ISSN:	1064-3745
Last Modified:	05 Nov 2024 11:15
URI:	https://orca.cardiff.ac.uk/id/eprint/173459

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