Assessing and Engineering Antibody Stability Using Experimental and Computational Methods

Zhang, Cheng and Dalby, Paul Anthony 2022. Assessing and Engineering Antibody Stability Using Experimental and Computational Methods. Methods in Molecular Biology 2552 , pp. 165-197. 10.1007/978-1-0716-2609-2_9

Full text not available from this repository.

Abstract

Engineering increased stability into antibodies can improve their developability. While a range of properties need to be optimized, thermal stability and aggregation are two key factors that affect the antibody yield, purity, and specificity throughout the development and manufacturing pipeline. Therefore, an ideal goal would be to apply protein engineering methods early-on, such as in parallel to affinity maturation, to screen out potential drug molecules with the desired conformational and colloidal stability. This chapter introduces our methods to computationally characterize an antibody Fab fragment, propose stabilizing variants, and then experimentally verify these predictions.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Biosciences
Publisher:	Springer
ISSN:	1064-3745
Last Modified:	28 Nov 2024 11:01
URI:	https://orca.cardiff.ac.uk/id/eprint/173705

Actions (repository staff only)

Edit Item