A novel multilayer antimicrobial urinary catheter material with antimicrobial properties

Gambrill, Benjamin, Pertusati, Fabrizio ORCID: https://orcid.org/0000-0003-4532-9101, Shergill, Iqbal, Hughes, Stephen and Prokopovich, Polina ORCID: https://orcid.org/0000-0002-5700-9570 2024. A novel multilayer antimicrobial urinary catheter material with antimicrobial properties. Materials Advances 10.1039/d4ma01045k

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Abstract

Urinary catheters are commonly used in medical practice to drain and monitor urine of patients. However, urinary catheterisation is associated with the risk of developing catheter-associated urinary tract infections (CAUTIs), which can result in life-threatening sepsis that requires antibiotics for treatment. Using the layer-by-layer (LbL) technique, we assembled a multilayer catheter comprising nine quadruple layers (9QL) of alginate, chlorhexidine (CHX), alginate and poly(β-amino ester) (PBAE) built upon an amino-functionalised silicone. The prepared catheter materials were tested for pre-packaged storage conditions and sterilisation techniques. The daily release of CHX was measured at pH 7.4 and pH 5 and simulated urine at 37 °C, which was used to determine the antimicrobial affect. CHX release was detected for a minimum of 14 days in PBS (pH 7.4), pH 5 release media, and simulated urine for the samples tested against storage conditions and sterilisation techniques. Incubation of the prepared material with bacterial cultures for 24 hours restricted bacterial growth compared to incubation with the standard material. The minimum inhibition concentration of CHX for clinically isolated urinary tract infection (UTI) bacterial strains was in the range of 19.4–77.4 µM, at which the released CHX could indirectly prevent bacterial growth for up to 14 days. Based on the daily CHX release from the samples, the hydrolysis of PBAE at pH 5 was gradual, resulting in a greater number of days of preventing bacterial growth, followed by pH 7.4 and then simulated urine. To the best of our knowledge, this is the first report on the use of PBAE in association with a urinary catheter material for the release of an antimicrobial drug.

Item Type:	Article
Date Type:	Published Online
Status:	In Press
Schools:	Pharmacy
Additional Information:	License information from Publisher: LICENSE 1: URL: http://creativecommons.org/licenses/by/3.0/, Start Date: 2024-12-28
Publisher:	Royal Society of Chemistry
Date of First Compliant Deposit:	16 January 2025
Date of Acceptance:	21 December 2024
Last Modified:	16 Jan 2025 12:30
URI:	https://orca.cardiff.ac.uk/id/eprint/175325

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