Analyzing DNA methyltransferase enzymatic activity: Basic methods and applications

Jurkowska, Renata Z. ORCID: https://orcid.org/0000-0002-4507-2222 2020. Analyzing DNA methyltransferase enzymatic activity: Basic methods and applications. Tollefsbol, Trygve, ed. Epigenetics Methods, Vol. 18. Translational Epigenetics Series, Academic Press, pp. 237-259. (10.1016/B978-0-12-819414-0.00012-4)

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Abstract

DNA methylation plays crucial biological roles in both prokaryotes and eukaryotes and is often dysregulated in diseases. DNA methyltransferases (MTases), enzymes transferring the methyl group to cytosine or adenine bases in DNA, have therefore been an object of intensive studies since their discovery. Hence, methods allowing investigation of the molecular properties of these fascinating enzymes are constantly being developed. Additionally, as DNA MTases are considered attractive therapeutic targets for human diseases, major efforts are made to identify potent and specific compounds targeting these key enzymes. Efficient inhibitor screening requires development of sensitive, highly accurate and easily scalable assay formats. This chapter focuses on the most common assays used for the detection of DNA MTase activity in cells and in vitro, and for studying their molecular mechanism and specificity. In addition, novel fluorescence- and electrochemical sensing-based assays well suited for compounds screening will be summarized, followed by the description of exciting new biosensors for real-time tracing of DNA methylation in cells.

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences
Publisher:	Academic Press
ISBN:	978-0128194140
Date of First Compliant Deposit:	17 July 2025
Last Modified:	21 Jul 2025 08:49
URI:	https://orca.cardiff.ac.uk/id/eprint/179887

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