Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas

Parfitt, Geraint ORCID: https://orcid.org/0000-0002-8704-7906, Pinali, Christian, Akama, Tomoya O., Young, Robert David ORCID: https://orcid.org/0000-0002-8300-8002, Nishida, Kohji, Quantock, Andrew James ORCID: https://orcid.org/0000-0002-2484-3120 and Knupp, Carlo ORCID: https://orcid.org/0000-0001-9127-2252 2011. Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas. Journal of Structural Biology 174 (3) , pp. 536-541. 10.1016/j.jsb.2011.03.015

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Abstract

The spatial distribution of collagen fibrils in the corneal stroma is essential for corneal transparency and is primarily regulated by extrafibrillar proteoglycans, which are multi-functional polymers that interact with hybrid type I/V collagen fibrils. In order to understand more about proteoglycan organisation and collagen associations in the cornea, three-dimensional electron microscopy reconstructions of collagen-proteoglycan interactions in the anterior, mid and posterior stroma from a Chst5 knockout mouse, which lacks a keratan sulphate sulphotransferase, were obtained. Both longitudinal and transverse section show sinuous, oversized proteoglycans with near-periodic, orthogonal off-shoots. In many cases, these proteoglycans traverse over 400 nm of interfibrillar space interconnecting over 10 collagen fibrils. The reconstructions suggest that multiple chondroitin sulphate/dermatan sulphate proteoglycans have aggregated laterally and, possibly, end-to-end, with orthogonal extensions protruding from the main electron-dense stained filament. We suggest possible mechanisms as to how sulphation differences may lead to this increase in aggregation of proteoglycans in the Chst5-null mouse corneal stroma and how this relates to proteoglycan packing in healthy corneas.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Subjects:	R Medicine > RE Ophthalmology
Uncontrolled Keywords:	Mouse cornea ; Collagen fibrils ; Proteoglycans ; Chst5 ; Three-dimensional reconstruction ; Electron microscopy
Publisher:	Elsevier
ISSN:	1047-8477
Last Modified:	19 Oct 2022 08:32
URI:	https://orca.cardiff.ac.uk/id/eprint/18116

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