Alamir, Hanin, Wong, Carissa C. W., Alsubaiti, Amal, Edmunds, Grace L., Alismail, Maryam, Huynh, Lan, Shi, Yiwei, Lewis, Philip A., Grant, Tressan, Alsulaimani, Safaa, Boyd, James, Holland, Christopher J., Morgan, David J., Gallimore, Awen M.  ORCID: https://orcid.org/0000-0001-6675-7004 and Wülfing, Christoph
2025.
TIM3 is a context-dependent coregulator of cytotoxic T cell function.
Science Signaling
18
(905)
, eadk4594.
10.1126/scisignal.adk4594
|
Abstract
TIM3 is a coregulatory receptor that is highly abundant on multiple immune cell types, including T cells in response to prolonged exposure to antigen, and it marks functionally suppressed cytotoxic T lymphocytes (CTLs) in the tumor microenvironment. TIM3 exhibits inhibitory function in vivo but paradoxically has costimulatory T cell signaling capability in vitro. Here, we found that TIM3 directly inhibited the function of murine and human CTLs in direct interaction with target tumor cell spheroids. TIM3 regulated the ability of suppressed CTLs to polarize their actin cytoskeleton as a required step in cytolysis. Whereas the expression of the proposed TIM3 ligands CEACAM1 and galectin 9 in trans on target tumor cells enhanced TIM3 function, expression of CEACAM1 in cis on CTLs had the opposite effect. TIM3 functioned as an inhibitory receptor on spheroid-suppressed CTLs but not on active CTLs in a two-dimensional tissue culture model. Together, these data suggest that TIM3 enhances T cell function, serving as either a coinhibitory or costimulatory receptor depending on the functional context of the T cell on which it is expressed.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Publisher: | American Association for the Advancement of Science |
| ISSN: | 1945-0877 |
| Date of Acceptance: | 4 September 2025 |
| Last Modified: | 07 Oct 2025 13:01 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/181529 |
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