Lysosomal pH measurements via dual-imaging microscopy

Kirkham, Emily, Gardiner, Sian, Lloyd-Evans, Emyr ORCID: https://orcid.org/0000-0002-3626-1611, Best, Hannah and Waller-Evans, Helen ORCID: https://orcid.org/0000-0003-4133-6064 2025. Lysosomal pH measurements via dual-imaging microscopy. Hughes, Stephanie M. and Basak, Indranil, eds. Lysosomes: Methods and Protocols, Vol. 2976. Methods in Molecular Biology, New York, New York, USA: pp. 11-24. (10.1007/978-1-0716-4844-5_2)

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Abstract

Dual-wavelength ratiometric fluorescent probes are the current gold standard for generating a quantitative measure of subcellular compartment ion contents. This chapter describes the use of fluorescent probes to measure lysosomal pH, encompassing probe selection, calibration, instrumentation, and the miniaturization of assays to use for high-throughput readouts.

Item Type:	Book Section
Date Type:	Publication
Schools:	Schools > Biosciences
ISBN:	978-1-0716-4843-8
Last Modified:	30 Oct 2025 11:45
URI:	https://orca.cardiff.ac.uk/id/eprint/181995

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