Spermine attenuates carotid body glomus cell oxygen sensing by inhibiting L-type Ca2+ channels

Cayzac, S. H., Rocher, A., Obeso, A., Gonzalez, C., Riccardi, Daniela ORCID: https://orcid.org/0000-0002-7322-3163 and Kemp, Paul J. ORCID: https://orcid.org/0000-0003-2773-973X 2011. Spermine attenuates carotid body glomus cell oxygen sensing by inhibiting L-type Ca2+ channels. Respiratory Physiology & Neurobiology 175 (1) , pp. 80-89. 10.1016/j.resp.2010.09.009

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Abstract

An increase in intracellular Ca2+ is crucial to O2 sensing by the carotid body. Polyamines have been reported to modulate both the extracellular Ca2+-sensing receptor (CaR) and voltage-gated Ca2+ channels in a number of cell types. Using RT-PCR and immunohistochemistry, the predominant voltage-gated Ca2+ channels expressed in the adult rat carotid body were L (CaV1.2) and N (CaV2.2)-type. CaR mRNA could not be amplified from carotid bodies, but the protein was expressed in the nerve endings. Spermine inhibited the hypoxia-evoked catecholamine release from isolated carotid bodies and attenuated the depolarization- and hypoxia-evoked Ca2+ influx into isolated glomus cells. In agreement with data from carotid body, recombinant CaV1.2 was also inhibited by spermine. In contrast, the positive allosteric modulator of CaR, R-568, was without effect on hypoxia-induced catecholamine release from carotid bodies and depolarization-evoked Ca2+ influx into glomus cells. These data show that spermine exerts a negative influence on carotid body O2 sensing by inhibiting L-type Ca2+ channels.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	carotid body; hypoxia; L-type calcium channels; polyamines; calcium-sensing receptor
Publisher:	Elsevier
ISSN:	1569-9048
Last Modified:	19 Oct 2022 09:05
URI:	https://orca.cardiff.ac.uk/id/eprint/19925

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