Glutathione S-transferases as molecular markers of tumour progression and prognosis in renal cell carcinoma

Searchfield, Lydia ORCID: https://orcid.org/0000-0001-9640-9326, Price, Sally, Betton, Graham, Jasani, Bharat, Riccardi, Daniela ORCID: https://orcid.org/0000-0002-7322-3163 and Griffiths, David Francis Rees 2011. Glutathione S-transferases as molecular markers of tumour progression and prognosis in renal cell carcinoma. Histopathology 58 (2) , pp. 180-190. 10.1111/j.1365-2559.2010.03733.x

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Abstract

Aims: Renal cell carcinoma (RCC) often recurs as distant metastasis; there is thus a need for new indicators to identify high-risk patients. Glutathione S-transferases (GST)- and -π are involved in the renal bioactivation of toxic metabolites. The aim was to investigate whether their expression is of diagnostic and prognostic value. Methods and results: Western blotting of microdissected normal kidney and immunostaining of histological RCC microarrays shows expression of GST- in proximal tubular cells, while GST-π was found in the distal nephron. Of the primary 174 RCC cases examined, GST- immunoreactivity was restricted to conventional RCC (n = 76, 68% positive) and was not seen in any other RCC subtypes. The cross-tabulation of the GST- scores with other prognostic indices demonstrated that GST- immunostaining was significantly more frequent in low-grade tumours (χ2: P < 0.004), and that conventional GST--positive RCC patients had a mean disease-free survival of 6.0 years (95% confidence interval 5.33–6.63), compared with 4.7 years (3.54–5.90) in GST--negative tumours (Kaplan–Meier survival analysis, P = 0.011, log-rank test). Conclusions: GST- is a highly specific diagnostic marker for primary conventional RCC, where it is a prognostic marker if grade is omitted from the multivariate analysis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General)
Uncontrolled Keywords:	3,3′-diaminobenzidine; glutathione S-transferases; horseradish peroxidase; immunohistochemistry; prognostic indicators; renal cell carcinoma; tissue microarray; Western blotting
Publisher:	Wiley
ISSN:	0309-0167
Last Modified:	29 Aug 2024 01:08
URI:	https://orca.cardiff.ac.uk/id/eprint/23049

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