Bubeck, Doryen, Roversi, Pietro, Donev, Rossen Mintchev, Morgan, Bryan Paul  ORCID: https://orcid.org/0000-0003-4075-7676, Llorca, Oscar and Lea, Susan M.
2011.
Structure of human complement C8, a precursor to membrane attack.
Journal of Molecular Biology
405
(2)
, pp. 325-330.
10.1016/j.jmb.2010.10.031
|
Abstract
Complement component C8 plays a pivotal role in the formation of the membrane attack complex (MAC), an important antibacterial immune effector. C8 initiates membrane penetration and coordinates MAC pore formation. High-resolution structures of C8 subunits have provided some insight into the function of the C8 heterotrimer; however, there is no structural information describing how the intersubunit organization facilitates MAC assembly. We have determined the structure of C8 by electron microscopy and fitted the C8α-MACPF (membrane attack complex/perforin)-C8γ co-crystal structure and a homology model for C8β-MACPF into the density. Here, we demonstrate that both the C8γ protrusion and the C8α-MACPF region that inserts into the membrane upon activation are accessible.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine > RB Pathology |
| Uncontrolled Keywords: | C8, complement, 3D electron microscopy, terminal pathway, membrane attack complex (MAC) |
| Publisher: | Elsevier |
| ISSN: | 0022-2836 |
| Last Modified: | 20 Oct 2022 07:40 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/26020 |
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