Cyclical expression of L-selectin (CD62L) by recirculating T cells

Klinger, A., Gebert, A., Bieber, K., Kalies, K., Ager, Ann ORCID: https://orcid.org/0000-0002-5763-8908, Bell, E. B. and Westermann, J. 2009. Cyclical expression of L-selectin (CD62L) by recirculating T cells. International Immunology 21 (4) , pp. 443-455. 10.1093/intimm/dxp012

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Abstract

L-Selectin (CD62L) mediates T-cell entry into lymph nodes. Whether the microenvironment modulates L-selectin expression of T cells during diapedesis and transit is unknown. Therefore, L-selectin expression was determined quantitatively on circulating T cells in blood, lymph nodes and thoracic duct by confocal laser scanning microscopy. We show that in contrast to leukocyte function-associated antigen-1 (CD11a/CD18) and ICAM-1 (CD54), L-selectin expression is cyclically expressed on recirculating T cells. It is reduced to ∼30% of the blood value during entry across high endothelial venules. Within lymph nodes, CD4+ T-cell subsets maintain reduced L-selectin expression at a similar level in all compartments (T-cell zone, B-cell zone and medulla). After exit, L-selectin is re-expressed to levels comparable to those of T cells in blood. Apparently, L-selectin levels are not only down-regulated during T-cell activation but also routinely reduced while transmigrating within lymph nodes. L-Selectin down-regulation seems to be ligand independent since it also occurs in the white pulp compartments of the spleen which lack classic L-selectin ligands such as GlyCAM-1 and CD34. In addition, T cells in non-lymphoid organs do not reveal reduced L-selectin levels. Thus, the ability of secondary lymphoid organs to reduce L-selectin expression of T cells prior to activation might be a prerequisite for their characteristic property to induce primary immune responses.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	CD62L, CLSM, ICAM-1, image analysis, LFA-1, L-selectin, lymph node, migration, organ compartments, rat, T cell
Publisher:	Oxford University Press
ISSN:	0953-8178
Last Modified:	20 Oct 2022 07:53
URI:	https://orca.cardiff.ac.uk/id/eprint/26825

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