RUNX1-ETO deregulates the proliferation and growth factor responsiveness of human hematopoietic progenitor cells downstream of the myeloid transcription factor, MYCT1 [Letter]

Liddiard, Kate ORCID: https://orcid.org/0000-0002-0953-1997, Burnett, Alan Kenneth, Darley, Richard Lawrence ORCID: https://orcid.org/0000-0003-0879-0724 and Tonks, Alex ORCID: https://orcid.org/0000-0002-6073-4976 2012. RUNX1-ETO deregulates the proliferation and growth factor responsiveness of human hematopoietic progenitor cells downstream of the myeloid transcription factor, MYCT1 [Letter]. Leukemia 26 (1) , pp. 177-179. 10.1038/leu.2011.188

Preview

PDF Download (244kB) | Preview

Abstract

To elucidate mechanisms by which RUNX1–ETO, the common acute myeloid leukemia t(8;21) fusion protein, primes hematopoietic cells for oncogenic transformation, we previously carried out Affymetrix gene expression profiling of RUNX1–ETO-expressing human CD34+ progenitor cells. We identified the MYC target gene, MYCT1, as a gene significantly upregulated at an early developmental stage that could be integral to RUNX1–ETO-mediated leukemic phenotype. To determine whether increased MYCT1 expression recapitulates the RUNX1–ETO phenotype, we performed retroviral transduction of MYCT1 as a single molecular abnormality into hematopoietic cell lines and normal human cord blood-derived CD34+ progenitor cells. In the absence of commercially available MYCT1 antibody, retroviral expression vectors, containing full-length MYCT1 coding sequence with (MYCT1-HA) or without (MYCT1) the addition of a C-terminal HA antigen tag, were also generated (Supplementary Materials and methods and Supplementary Figure S1), and transgenic MYCT1 over-expression was confirmed at the mRNA and protein levels (Supplementary Figure S2). Functional validation (Supplementary Figure S3) was provided by replication of the reduced viability of MYCT1-expressing murine myeloid 32D cells in the absence of WEHI-3B-conditioned medium reported by Yin et al.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Publisher:	Nature Publishing Group
ISSN:	0887-6924
Last Modified:	13 May 2023 15:36
URI:	https://orca.cardiff.ac.uk/id/eprint/26951

Citation Data

Cited 4 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item