Simian immunodeficiency virus SIVmac239 nef vaccination elicits different tat28-35SL8-specific CD8+ T-cell clonotypes compared to a DNA prime/adenovirus type 5 boost regimen in rhesus macaques

Burwitz, Benjamin J., Ende, Zachary, Sudolcan, Benjamin, Reynolds, Matthew, Greene, Justin M., Bimber, Benjamin N., Almeida, Jorge R., Kurniawan, Monica, Venturi, Vanessa, Gostick, Emma, Wiseman, Roger W., Douek, Daniel C., Price, David ORCID: https://orcid.org/0000-0001-9416-2737 and O'Connor, David H. 2011. Simian immunodeficiency virus SIVmac239 nef vaccination elicits different tat28-35SL8-specific CD8+ T-cell clonotypes compared to a DNA prime/adenovirus type 5 boost regimen in rhesus macaques. Journal of Virology 85 (7) , pp. 3683-3689. 10.1128/JVI.02112-10

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Abstract

Different human immunodeficiency virus (HIV)/simian immunodeficiency virus (SIV) vaccine vectors expressing the same viral antigens can elicit disparate T-cell responses. Within this spectrum, replicating variable vaccines, like SIVmac239Δnef, appear to generate particularly efficacious CD8+ T-cell responses. Here, we sequenced T-cell receptor β-chain (TRB) gene rearrangements from immunodominant Mamu-A*01-restricted Tat28-35SL8-specific CD8+ T-cell populations together with the corresponding viral epitope in four rhesus macaques during acute SIVmac239Δnef infection. Ultradeep pyrosequencing showed that viral variants arose with identical kinetics in SIVmac239Δnef and pathogenic SIVmac239 infection. Furthermore, distinct Tat28-35SL8-specific T-cell receptor (TCR) repertoires were elicited by SIVmac239Δnef compared to those observed following a DNA/Ad5 prime-boost regimen, likely reflecting differences in antigen sequence stability.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QR Microbiology
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0022-538X/ (accessed 25/02/2014)
Publisher:	American Society for Microbiology
ISSN:	0022-538X
Date of First Compliant Deposit:	30 March 2016
Last Modified:	20 May 2023 19:10
URI:	https://orca.cardiff.ac.uk/id/eprint/27494

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